Seedling cultivation of winter jujube seedlings. For donor collection, jujube trees used for tissue culture are called donors. Tissue culture must be a good mother plant. Take the old jujube head or the lychee jujube head of the good mother plant, and use the shoots from the annual jujube hydroponic culture as the propagation material. For material processing, the tissue culture material is first rinsed under tap water, then sterilized with 0.1% potassium permanganate disinfectant or 0.1% liters of mercury, followed by sterile water for 4 to 5 times. The fully sterilized material is subdivided into 2 m long stem segments or shoot tips under sterile conditions and inoculated on sterile medium. The medium is selected. The suitable basic mediums selected from the tissue culture of winter jujube seedlings are MS, H, WP, N6, etc. On this basis, BA, KT, NAA, IBA and other hormones are added in appropriate amounts. A starter medium, a differentiation medium, and a rooting medium; the differentiation medium and the rooting medium may also be combined into one medium. Under the environmental conditions, the temperature of jujube tissue culture is 28±2°C, the illumination is mainly glare, the intensity is 2000～4000lx, and the illumination time is 10～12h/d. When the test tube seedlings are transplanted, when the height of the seedlings in the bottle reaches 5 to 7 cm, the bottle can be transplanted when the leaves are green. Open the bottle stopper before the bottle is opened for 3 days to 4 days. Wash the root culture medium when transplanting winter jujube seedlings, and disinfect with 0.1% potassium permanganate to prevent rotten roots after planting. In the greenhouse, the test tube seedlings are first moved into the greenhouse to refine the seedlings. The seedbed bed is mixed with vermiculite, river sand, perlite, etc., or mixed with the sandy loam, and the soil is disinfected before use. Planting density is 5~10cm, watering immediately after planting, maintaining the necessary temperature and humidity. Spray sterilizing agent once every other week